Ensure the bitstream properties and flash programming file options were implemented correctly for the targeted configuration mode. To verify the bitstream generation options used by an image, run the Tcl command:
report_property -all [current_design]
This command will display all properties applied to a design. Where there are no values displayed, the default is applied. Also, review the flash programming file generation options. Verify that the proper data widths and data ordering options are used for the flash programming file generation. All DRC warnings received during configuration file generation should be reviewed and corrected.