The VIO v2.0 core has a feature that allows you to specify an initial value for each output probe port. You can reset the VIO core output probe ports to these initial values by right-clicking the VIO core in the Hardware window and selecting the Reset VIO Core Outputs option. You can also reset the VIO core outputs using a Tcl command:
reset_hw_vio_outputs [get_hw_vios {hw_vio_1}]
Note: Resetting the VIO output probes to their initial
values cause the output probe values to become out-of-sync with the Vivado IDE. Refer to the section called Synchronizing the VIO Core Output Values
to the Vivado IDE on how to handle this situation.